top of page
Fashion with Mask

What is RT-PCR ?

Man with Clipboard
PCR test
Science Lab

What is RT-PCR?

RT-PCR (reverse transcription-polymerase chain reaction) is a laboratory method used to make many copies of a specific genetic sequence for analysis. It uses an enzyme called reverse transcriptase to change a specific piece of RNA into a matching piece of DNA. This piece of DNA is then amplified (made in large numbers) by another enzyme called DNA polymerase. The amplified DNA copies help tell whether a specific mRNA molecule is being made by a gene. RT-PCR may be used to look for certain changes in a gene or chromosome or for activation of certain genes, which may help diagnose a disease, such as cancer. It may also be used to study the RNA of certain viruses, such as the human immunodeficiency virus (HIV) and the hepatitis C virus, to help diagnose and monitor infection. 

Having been around since 1983, RT-PCR  is the most sensitive technique for quantitative and mRNA detection currently available and is considered the “gold standard” for test results.

Feel free to contact us with any question

How does RT-PCR identify SARS-CoV-2 COVID-19?

To aid in the fight against COVID-19 transmission, the ATILA BioSystems© iAMP COVID-19 Detection Kit was developed to utilize the RT-PCR methodology in testing for the qualitative detection of nucleic acid from SARS-CoV-2 in upper and lower respiratory specimens (nasopharyngeal or oropharyngeal swabs) collected from individuals suspected of COVID-19 by their healthcare provider, as well as upper respiratory specimens collected from any individual, including individuals without symptoms or other reasons to suspect COVID19.

bottom of page